KMID : 0545120180280010157
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Journal of Microbiology and Biotechnology 2018 Volume.28 No. 1 p.157 ~ p.164
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Humanized Mice for the Evaluation of Francisella tularensis Vaccine Candidates
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Oh Han-Seul
Kim C-Yoon Kim Chang-Hwan Hur Gyeung-Haeng Lee Ji-Min Chang Seo-Na Park Jae-Hak
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Abstract
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Francisella tularensis (FT), a highly infectious pathogen, is considered to be a potential biological weapon owing to the current lack of a human vaccine against it. Tul4 and FopA, both outer membrane proteins of FT, play an important role in the bacterium¡¯s immunogenicity. In the present study, we evaluated the immune response of mice? humanized with human CD34+ cells (hu-mice)?to a cocktail of recombinant Tul4 and FopA (rTul4 and rFopA), which were codon-optimized and expressed in Escherichia coli. Not only did the cocktail-immunized hu-mice produce a significant human immunoglobulin response, they also exhibited prolonged survival against an attenuated live vaccine strain as well as human T cells in the spleen. These results suggest that the cocktail of rTul4 and rFopA had successfully induced an immune response in the hu-mice, demonstrating the potential of this mouse model for use in the evaluation of FT vaccine candidates.
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KEYWORD
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Francisella tularensis, humanized mice, vaccine
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